Primer3 Input Site

If you have ever used an online primer design tool, chances are you were using under the hood. Written in C and later wrapped in Python (Primer3-py) or web interfaces (Primer3Plus), this engine is the gold standard for picking oligonucleotides.

PRIMER_OPT_TM=60.0 PRIMER_MIN_TM=58.0 PRIMER_MAX_TM=62.0 PRIMER_MAX_DIFF_TM=1.5 primer3 input

SEQUENCE_INTERNAL_OLIGO_MIN_SIZE=18 SEQUENCE_INTERNAL_OLIGO_OPT_TM=55.0 Save this as my_primers.txt : If you have ever used an online primer